Electron microscopic immunohistochemical identification of endothelial cells in the rabbit.
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چکیده
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Electron microscopic immunohistochemical identification of endothelial cells in the rabbit.
Antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. The distribution of the peroxidase marker then was observed by electron microscopy. We examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut l...
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Electron micrographs were obtained showing the individual, shadow-cast macromolecules from solutions of purified anti-p-azobenzoate rabbit antibody and of normal gamma-globulin. The two materials look alike and consist mainly of asymmetrical rod-like particles about 30 to 40 A in diameter. Lengths are not constant but the weight average is about 250 A for the antibodies and about 200 A for the ...
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Presence of glial cells in the rat pineal gland: a light and electron microscopic immunohistochemical study.
Immunoperoxidase methods for the demonstration of three glial antigens, vimentin, glial fibrillary acidic protein, and S-100 protein, were applied to routine-fixed paraffin sections of rat pineal gland. A pre-embedding electron microscope immunoperoxidase method was also used to study the ultrastructural localization of S-100 protein in pineal cells. Light and electron microscopic results showe...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1976
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.38.3.146